Centri-Sep 96 filter plates have been developed and optimized to remove the excess dye terminators from the extension products in the Big Dye®Terminator cycle sequencing reactions. Sample volumes of 5 to 20 microliters are efficiently purified in less than 10 minutes. Purified samples result in clean electrophoretic results and long base reads. Transferring the samples to the plates is an extremely important part of the procedure and is essential to getting good quality sequencing results.